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Part:BBa_K3947005

Designed by: David Dimech   Group: iGEM21_MSP-Maastricht   (2021-10-19)


Spacer System

The Forward Spacer Contains 29 nucleotides of space, along with a Ribosome Binding Site which originated from plasmid pET 39b(+). This would be placed before the gene of interest.

The Back Spacer contains a 6x Histidine tag, for protein purification and testing. This one will be placed after the gene, the histidine tag has to be place in frame with the protein. Contains multiple stop codons to ensure that translation has stopped.

Sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 31
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None